Description
>The Boster AlamarBlue Cell Viability Assay Reagent is used to quantitatively measure the viability of mammalian cell lines, bacteria and fungi by incorporating a rapid, sensitive and reliable fluorometric/colorimetric growth indicator. AlamarBlue detects cell viability by converting from a non-fluorescent dye to the highly red fluorescent dye resorufin in response to chemical reduction of growth medium resulting from cell growth. The assay is homogenous, requires no cell lysis or washing and offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. The AlamarBlue Reagent allows for viability studies of chemical inhibitors on tumor cell lines and toxicology research to establish baseline data for predicting the toxicity of related novel agents by comparing such baseline data with known in vivo toxicity. Viable cells maintain a reducing environment within their cytoplasm. The AlamarBlue Reagent is an oxidized form of redox indicator that is blue in color and non-fluorescent. When incubated with viable cells, the reagent changes color from blue to red and becomes fluorescent (Figure 1 in datasheet). This change can be detected using fluorescence or absorbance measurement (Figure 2 in datasheet)